In Vitro Incubation of Gills Isolated from the Gulf Killfish, Fundulus Grandis

Comparing Methods of Euthanasia and Gill Culture for Hypoxia Research on the Gulf Killifish, Fundulus grandis

Contemporary and historical influences on the genetic structure of the estuarine-dependent Gulf killifish Fundulus grandis

In comparison to species living in open marine environments, estuarine-dependent species are expected to exhibit stronger genetic population structure due to dispersal limitations. Estuarine habitats are relatively transitory on geological time scales; thus, populations may not be at migration–drift equilibrium, which could confound estimates of current day gene flow or selection. We used 8 nuc...

Effects of long-term hypoxia on enzymes of carbohydrate metabolism in the Gulf killifish, Fundulus grandis.

The goal of the current study was to generate a comprehensive, multi-tissue perspective of the effects of chronic hypoxic exposure on carbohydrate metabolism in the Gulf killifish Fundulus grandis. Fish were held at approximately 1.3 mg l(-1) dissolved oxygen (approximately 3.6 kPa) for 4 weeks, after which maximal activities were measured for all glycolytic enzymes in four tissues (white skele...

Is Exposure to Macondo Oil Reflected in the Otolith Chemistry of Marsh-Resident Fish?

Genomic and physiological responses in Gulf killifish (Fundulus grandis) in the northern Gulf of Mexico have confirmed oil exposure of resident marsh fish following the Macondo blowout in 2010. Using these same fish, we evaluated otolith microchemistry as a method for assessing oil exposure history. Laser-ablation inductively-coupled-plasma mass spectrometry was used to analyze the chemical com...

Microinjection of chemical carcinogens into small fish embryos: exocrine pancreatic neoplasm

Embryos of gulf killifish Fundulus grandis were injected with N-methyl-N'-nitro-Nnitrosoguanidine (MNNG). Retention of carcinogen in the egg immediately after injection was highly variable. Hatching percentages of MNNG-injected and uninjected embryos were similar, but survival of MNNG-injected fish during the first week after hatching was less than half of the survival of controls. One of 3 MNN...